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Proteintech twist1
Twist1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+twist/TWIST1-specific+Antibody/pm41828479-238-78-81
Average 96 stars, based on 145 article reviews
twist1 - by Bioz Stars, 2026-10
96/100 stars

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other:

Article Title: NF- κ B Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner.
Article Snippet: The following antibodies were used: anti-GAPDH (60004-1-Ig, Proteintech), anti-Ecadherin (14472, Cell Signaling Technology), anti-Snail-1 (13099-1-AP, Proteintech), anti-Slug (GTX128796, GeneTex), anti-p65 (sc-8008, Santa Cruz), anti-p-p65 (sc136548, Santa Cruz), anti-phospho-IκB-α (2859, Cell Signaling Technology), anti-IκB-α (CSB-RA015761A0HU, Cusabio Technology), anti-SLC7A11 (NB300-318, Novus), antiβ-catenin (phospho Y142, ab27798, Abcam), anti-β-catenin (ab32572, Abcam), anti-Cyclin-D1 (60186-1-Ig, Proteintech), anti-Bad (9292, Cell Signaling Technology), anti-Bcl2 (15017, Cell Signaling Technology), anti-Vimentin (GB111308, Servicebio), anti-Twist (25465-1-AP, Proteintech), anti-ZEB1 (21544-1-AP, Proteintech), anti-Nrf2 (M200-3, Medical and Biological Laboratories), anti-TFR1 (ab214039, Abcam), and anti-GPX4 (67763-1-Ig, Proteintech).

Article Title: TCF4 and HuR Mediated-METTL14 Suppresses Dissemination of Colorectal Cancer via N6-Methyladenosine-Dependent Silencing of ARRDC4
Article Snippet: Immunoblots signals were detected using Pierce ECL Western Blotting Substrate (32106, Thermo Fisher Scienti c).

Article Title: NF- κ B Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner
Article Snippet: The following antibodies were used: anti-GAPDH (60004-1-Ig, Proteintech), anti-E-cadherin (14472, Cell Signaling Technology), anti-Snail-1 (13099-1-AP, Proteintech), anti-Slug (GTX128796, GeneTex), anti-p65 (sc-8008, Santa Cruz), anti-p-p65 (sc-136548, Santa Cruz), anti-phospho-I κ B- α (2859, Cell Signaling Technology), anti-I κ B- α (CSB-RA015761A0HU, Cusabio Technology), anti-SLC7A11 (NB300-318, Novus), anti- β -catenin (phospho Y142, ab27798, Abcam), anti- β -catenin (ab32572, Abcam), anti-Cyclin-D1 (60186-1-Ig, Proteintech), anti-Bad (9292, Cell Signaling Technology), anti-Bcl2 (15017, Cell Signaling Technology), anti-Vimentin (GB111308, Servicebio), anti-Twist (25465-1-AP, Proteintech), anti-ZEB1 (21544-1-AP, Proteintech), anti-Nrf2 (M200-3, Medical and Biological Laboratories), anti-TFR1 (ab214039, Abcam), and anti-GPX4 (67763-1-Ig, Proteintech).

Article Title: Cyclovirobuxine D Inhibits Triple-Negative Breast Cancer via YAP/TAZ Suppression and Activation of the FOXO3a/PINK1-Parkin Pathway-Induced Mitophagy
Article Snippet: Background: Triple-negative breast cancer (TNBC) is characterized by its rapid progression and aggressive nature, with limited effective therapeutic interventions currently available.. Cyclovirobuxine D (CVB-D), a natural alkaloid extracted from the traditional Chinese herb Buxus sinica, is renowned for its cardioprotective and anti-ischemic effects, demonstrating notable anti-cancer properties.. Nevertheless, the anti-tumor effects of CVB-D on TNBC

Incubation:

Article Title: Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog‐mediated epithelial‐mesenchymal transition
Article Snippet: Proteins were then electrotransferred to PVDF membranes (Millipore). .. Membranes were blocked in 10% skimmed milk and then incubated with primary Abs, including anti‐POU4F2 (1:1000; ab235268, Abcam), anti‐rabbit GAPDH (1:6000; 10494‐1‐AP, Proteintech), anti‐mouse GAPDH (1:6000; 60004‐1‐Ig, Proteintech,), anti‐E‐cadherin (1:1000; 20874‐1‐AP, Proteintech), anti‐vimentin (1:4000; 10366‐1‐AP, Proteintech), anti‐Slug (1:1000; 12129‐1‐AP, Proteintech), anti‐Twist (1:500;, 25465‐1‐AP, Proteintech), anti‐Snail1 (1:500; 13099‐1‐AP, Proteintech ), anti‐ZEB1 (1:2000; 21544‐1‐AP, Proteintech), anti‐ZEB2 (1:1000; 14026‐1‐AP, Proteintech), anti‐β‐actin (1:5000; 66009‐1‐Ig, Proteintech), anti‐SHH (1:800; 20697‐1‐AP, Proteintech), anti‐PTCH1 (1:500; ab53715, Abcam), anti‐SMO (1:2000; 66851‐1‐Ig, Proteintech), and anti‐GLI‐1 (1:3000; 66905‐1‐Ig, Proteintech) at 4°C overnight. .. Membranes were then incubated with HRP‐conjugated secondary Ab (anti‐mouse IgG [H + L] or anti‐rabbit IgG [H + L]; 1:10 000, 14 709, or 14 708; Cell Signaling Technology) at 25°C for 2 hours.

Membrane:

Article Title: Senescence cell signature associated with poor prognosis, epithelial-mesenchymal transition, solid histology, and spread through air spaces in lung adenocarcinoma.
Article Snippet: Cellular senescence is involved in critical processes in tumor progression.. Despite this potential relationship, the relationship between tumor cell senescence, prognostic significance, spread through air spaces (STAS), and tumor histology has not been investigated in lung adenocarcinoma (LUAD).. We used the LUAD PanCancer Atlas dataset to assess senescence cell signature (SCS) based on the SenMayo gene set.

Control:

Article Title: TCF4 and HuR mediated-METTL14 suppresses dissemination of colorectal cancer via N6-methyladenosine-dependent silencing of ARRDC4
Article Snippet: Immunoblots signals were detected using PierceTM ECL Western Blotting Substrate (32106, Thermo Fisher Scientific). .. Primary antibodies were listed as: anti-METTL14 (HPA038002, Sigma-Aldrich), anti-HuR (#12582, CST), anti-IGF2BP1 (22803-1-AP, Proteintech), anti-YTHDC1 (14392-1-AP, Proteintech), anti-YTHDF1 (17479-1-AP, Proteintech), anti-YTHDF2 (24744-1-AP, Proteintech), anti-YTHDF3 (25537-1-AP, Proteintech), anti-ARRDC4 (HPA042109, Sigma-Aldrich), anti-LaminB1 (66905-1-lg, Proteintech), anti-PSAT1 (A6707, ABclonal), anti-HSP90AA1 (A13501, ABclonal), anti-ANXA1 (A1118, ABclonal), anti-ZEB1 (21544-1-AP, Proteintech), anti-Snail (13099-1-AP, Proteintech), anti-Slug (#9585, CST), anti-Twist (25465-1-AP, Proteintech), anti-TCF4 (22337-1-AP, Proteintech), anti-GAPDH (60004-1-lg, Proteintech) or β-actin (66009-1-lg, Proteintech) were used as the internal control. .. Total RNA of cells was extracted by TRIzol Regent (Invitrogen) according to the previous protocols followed by cDNA synthesis with PrimeScriptTM RT Master Mix (RR036A, TAKARA). mRNA expression levels were measured by TB Green® Premix Ex TaqTM II Kit (RR820A, TAKAR) on Applied Biosystems StepOnePlus.

Western Blot:

Article Title: TTK Defines a High-Risk Oral Squamous Cell Carcinoma Subtype Through Dual mTORC1/NF-κB Activation
Article Snippet: .. Antibodies used in this study: Anti-TTK (Abcam, ab11108), Anti-mTOR (CST, #2983), Anti-p-mTOR (CST, #5536), Anti-p65 (Proteintech, 66535-1-Ig), Anti-p-p65 (CST, #3033), Anti-S6K (CST, #34475), Anti-p-S6K (CST, #9234), Anti-4EBP1 (#9644), Anti-p-4EBP1 (Santa Cruz Biotechnology, sc-293124), Anti-p38 (CST, #8690), Anti-p-p38 (CST, #4511), Anti-JNK (CST, #9252), Anti-p-JNK (CST, #4668), Anti-TAK1 (Abcam, ab109526), Anti-p-TAK1 (CST, #4508), Anti-TAB1 (Proteintech, 27566-1-AP), Anti-TAB3 (Abcam, ab124723), Anti-IKKα (Abcam, ab32041), Anti-IKKβ (Abcam, ab124957), Anti-p-IKKα/β (CST, #2697), Anti-IKBα (CST, #4812), Anti-p-IKBα (CST, #2859), Anti-c-Myc (Santa Cruz Biotechnology, sc-42), Anti-Cyclin D1 (Proteintech, 26939-1-AP), Anti-E-cadherin (Proteintech, 20874-1-AP), Anti-N-cadherin (Proteintech, 22018-1-AP), Anti-ZEB1 (Proteintech, 21544-1-AP), Anti-TWIST (Proteintech, 25465-1-AP), Anti-MMP2 (Proteintech, 10373-2-AP), Anti-rabbit IgG (Santa Cruz Biotechnology, sc-2004), Anti-mouse IgG (Santa Cruz Biotechnology, sc-2005), For Western blotting, the primary antibody was used at a dilution of 1:1000 and the secondary antibody at 1:2500. ..



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